The Specificity of Enzymes for Certain Substrates
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Cleavage of mispaired heteroduplex DNA substrates by numerous restriction enzymes.
The utility of restriction endonucleases as a tool in molecular biology is in large part due to the high degree of specificity with which they cleave well-characterized DNA recognition sequences. The specificity of restriction endonucleases is not absolute, yet many commonly used assays of biological phenomena and contemporary molecular biology techniques rely on the premise that restriction en...
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تاریخ انتشار 2013